Identification and Characterization of the Penicillin-Binding Protein 2a of Streptococcus pneumoniae and Its Possible Role in Resistance to b-Lactam Antibiotics
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Identification, purification, and characterization of transpeptidase and glycosyltransferase domains of Streptococcus pneumoniae penicillin-binding protein 1a.
Resistance to beta-lactam antibiotics in Streptococcus pneumoniae is due to alteration of penicillin-binding proteins (PBPs). S. pneumoniae PBP 1a belongs to the class A high-molecular-mass PBPs, which harbor transpeptidase (TP) and glycosyltransferase (GT) activities. The GT active site represents a new potential target for the generation of novel nonpenicillin antibiotics. The 683-amino-acid ...
متن کاملBiochemical characterization of Streptococcus pneumoniae penicillin-binding protein 2b and its implication in beta-lactam resistance.
Extensive use of beta-lactam antibiotics has led to the selection of pathogenic streptococci resistant to beta-lactams due to modifications of the penicillin-binding proteins (PBPs). PBP2b from Streptococcus pneumoniae is a monofunctional (class B) high-molecular-weight PBP catalyzing the transpeptidation between adjacent stem peptides of peptidoglycan. The transpeptidase domain of PBP2b isolat...
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II. The occurrence of modified penicillin-binding sites Modified PBPs have a lower affinity for β-lactam antibiotics, requiring clinically unattainable concentrations of the drug to effect its bactericidal activity Example: penicillin resistance in Streptococcus pneumoniae (pneumococcus) is caused by altered PBPs I. Production of β-lactamases This family of enzymes can inactivate penicillins by...
متن کاملAcquisition of five high-Mr penicillin-binding protein variants during transfer of high-level beta-lactam resistance from Streptococcus mitis to Streptococcus pneumoniae.
Penicillin-resistant isolates of Streptococcus pneumoniae generally contain mosaic genes encoding the low-affinity penicillin-binding proteins (PBPs) PBP2x, PBP2b, and PBP1a. We now present evidence that PBP2a and PBP1b also appear to be low-affinity variants and are encoded by distinct alleles in beta-lactam-resistant transformants of S. pneumoniae obtained with chromosomal donor DNA from a St...
متن کاملBiochemical Characterization of Streptococcus pneumoniae Penicillin-Binding Protein 2b and Its Implication in -Lactam Resistance
Biochemical Characterization of Streptococcus pneumoniae Penicillin-Binding Protein 2b and Its Implication in -Lactam Resistance Estelle Pagliero, Laurent Chesnel, Julie Hopkins, Jacques Croizé, Otto Dideberg, Thierry Vernet,* and Anne Marie Di Guilmi Laboratoire d’Ingénierie des Macromolécules, and Laboratoire de Cristallographie Macromoléculaire, Institut de Biologie Structurale Jean-Pierre E...
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تاریخ انتشار 2000